Composite

Part:BBa_K4167002:Design

Designed by: Xinzichu Xie   Group: iGEM22_ICJFLS   (2022-10-01)


Toehold switch-amilGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pET-28a-toehold switch-amilGFP plasmid was designed to comprise some antisense sequence of miRNA let-7d-3p, constant sequence, and part sequence of miRNA let-7d-3p, which form a toehold switch, as well as the gene of marker protein amilGFP. At the presence of miRNA let-7d-3p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of amilGFP, which is easily measured.


Source

miRNA let-7d-3p comes from human, amilGFP comes from Jelly fish.

References